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computer-assisted sperm analysis (casa) systems have evolved over approximately 40 years, through advances in devices to capture the image from a microscope, huge increases in computational power concurrent with amazing reduction in size of computers, new computer languages, and updatedexpanded software algorithms. Our study aimed to investigate the effect of season on functional parameters of frozen-thawed bovine semen using computer-assisted sperm analysis (casa) and flow cytometry. For this purpose, 86 ejaculates were collected from five mature holstein-friesian bulls kept under subtropical conditions during summer (august to september n 43) and winter (december to january n 43) months. A semen analysis (plural semen analyses), also called seminogram evaluates certain characteristics of a males semen and the sperm contained therein. It is done to help evaluate male fertility, whether for those seeking pregnancy or verifying the success of vasectomy. Depending on the measurement method, just a few characteristics may be evaluated (such as with a home kit) or many characteristics may be evaluated (generally by a diagnostic laboratory). At ivf1, we use the most advanced computer assisted semen analysis for the most accurate results. Casa is used to study recorded video images of sperm through an extreamly powerful microscope. The images are then digitized and analyzed by a high-speed computer system (up to 60 frames per second) so that motility of individual sperm (and sperm populations) can be analyzed accurately. Computerassisted sperm analysis systems offer an accurate, rapid and simultaneous assessment of different semen parameters such as concentration, total and progressive motility, slow, medium and rapid moving spermatozoa, linearity of sperm movement, the beat cross frequency, the amplitude of the lateral head displacement and different velocity parameters (verstegen et al.). Be predicted using manual assessment of sperm morphology and computer-assisted morphometric analysis did not add further information. Fertil steril 55989, 1991 our laboratory and others showed the importance of sperm morphology in predicting the outcome of received september 25, 1990 revised and accepted, january 4, 1991. Sca casa system for automatic analysis of concentration, motility, morphology, dna fragmentation, vitality and acrosome reaction analysis of semen samples.